Tris(hydroxymethyl)methylglycine CAS NO.5704-04-1

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Detailed Product Description

CAS:	5704-04-1	Assay:	99%
Formula:	C6H13NO5	Apearance:	White Crystalline Powder
MP/BP:	180-186℃	Storage:	Room Temperature

 

 

Tricine  Biological Buffer  Tris(hydroxymethyl)methylglycine CAS NO 5704-04-1

 

 

Quick detail:

 

Name	Tricine	Appearance	White crystalline powder
Assay,%	99.0-101.0	Water Content,%	≤1.0
PH(1% aq.)	4.6-5.3	Solubility(10% aq.)	Clear, colorless solution
Heavy Metals,ppm	≤5	Ultraviolet absorbance/260nm(0.1 M aqueous.)	≤0.04

 

Description:

> Formula:C6H13NO5

> Weight: 179.17

> MP/BP :180-186℃

> Storage:room temperature

> Safety Description:S26 S36/37/39

> Danger Description:R36/37/38

> Danger Symbol: Xi

 

 

Synonyms:

LABOTEST-BB LT00454966;n-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-glycin;TRICINE;TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE;TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE;TRIS-HCL;TRIS-HCL BUFFER;TRIS HYDROCHLORIDE;N-(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)glycine; N-[Tris(hydroxymethyl)methyl]glycine; Tricine N-Tris(hydroxymethyl)methylglycine; N-(Trishydroxymethylmethyl)glycine; N-(tri(hydroxymethyl)methyl)glycine; Tricine, Molecular Biology Grade N-Tris(hydroxymethyl)methylglycine, Molecular Biology Grade; Tricine, ULTROL Grade; N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine; LABOTEST-BB LT00454966; n-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]-glycin; TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE; TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE; TRIS-HCL BUFFER; N-[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]glycine

 

Related categories:Biochemistry;Good's Buffers;Buffer

 

Related Products:60-32-2

 

The determination of PH

Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, determination of the pH used have been calibrated pH meter.

 

The determination of solubility

Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, observe the solution.

 

The determination of KF

Use MA-1 intelligent Carle Fischer moisture tester to measure water content

 

The determination of UV absorbance

 A certain amount of the sample was weighed to the nearest 0.0001g, into 10ml volumetric flask, after constant volume with pure water, at a specific wavelength by 2cm cuvette was measured by UV spectrophotometer.

 

Detection of heavy metals

    The meaning of heavy metals of this act is under the specified experimental condition, which can coloration effects with sodium sulfide or thioacetamide  .The glasses of preparation or storage is not allowed to contain lead.

5.8.1  Reagents

  a) Lead dinitrate:1μg/mL

  b) Sodium hydroxide :10%(m/v)

  c) Sodium sulfide :10%(m/v)

5.8.2  Preparation of lead standard solution

    Weigh lead dinitrate  0.1599g ,relocated to 1000mL volumetric flask, plus nitric acid 5mL and water 50mL, diluted with water to the mark , shake, as the stock solution.

5.8.3 Determination of step

  Before use, the exact amount of stock solution 1mL, relocated to a 100ml volumetric flask, dilute with water to the mark, shake, limit the use of the day .

  Weigh sample 1g, after adding sodium hydroxide solution 5mL and 20mL of water to dissolve, set Nessler colorimetric tube, add 5 drops of sodium sulfide , shake , compared the color with 5mL lead standard solution after the same treatment,  not deeper.

 

Determination of melting point

Instrument:

Micro melting point apparatus WRS-2A, Melting point tubes

Determination of step:

Open switch, stable 20min.

First set the starting temperature than the melting point of the low 10 ℃, heating rate 2 ℃ / min.

The sample was grind as fine as possible and load in a clean, dry melting point pipe ,until the sample was co