DTT CAS NO.27565-41-9

Suzhou yacoo science co.,LTD was founded in 2003. It is a high-tech enterprise which specializing in biomedicine, electrochemical materials, import and export business, electronic mall, Up to now，It has 10 years history on chemical sales, R & D and production operations. Company also set sales and service outlets in Shanghai, Beijing, Guangzhou, Jinan and other cities, which owning first-class marketing team and technical support personnel. Its famous brands-Yacoo were exported to Europe, America and Southeast Asian countries. In order to meet the demand of the market of High-tech industrial products which updates fast, respones service timely, requires high technical and grows rapidly , Yacoo has established 5000㎡  research and test center and 10000㎡ production base， which have passed ISO9001: 2008 quality system certification. We have rapid logistics network, high quality service to provide  more than 20000 kinds of products for China and global customer. Relying on its own synthesis of unique and rich experience in research and development, provide scientific products from grams to tons of commissioned service as well as the mass production services for customers.

 

Detailed Product Description

CAS:	3483-12-3	Assay:	99%
Formula:	C4H10O2S2	MP/℃:	41.0-44.0
Appearance:	White Powder	Storage:	2-8℃

 

 

CAS NO 27565-41-9 ELISA Reagents DL-Dithiothreitol 99%

Quick Detail:

 

Name	DL-Dithiothreitol	Appearance	White  powder
Assay	≥99.0	Solubility(50mg/ml aq.)	Clear, colorless solution
Heavy Metals, ppm	≤5	PH(0.1M aq.)	4.0-6.5

 

 

Description:

> Formula: C4H10O2S2

> Weight:154.25

> Appearance:White  powder

> MP/℃:41.0-44.0

>Storage:2-8℃

> Safety Description:S26 S37/39

> Danger Description:R22 R36/37/38

> Danger Symbol:Xn

 

1.Applications:

  A. DTT acts as a cell permeable protective agent for Thiol groups..

  B. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE

  C.DTT is used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.

2.Location

  A. biology/biochemistry laboratory, pharmaceutical factory, cosmetics factory

3. Note:

  DTT is less pungent and is less toxic than 2-mercaptoethanol. It has been reported that a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol.

 

Specifications

Assay	≥99.0
MP/℃	41.0-44.0
Ultraviolet absorbance(0.1M aq.)	260nm:≤0.4 ;280nm:≤0.1
Heavy Metals, ppm	≤5
PH(0.1M aq.)	4.0-6.5
Shelf life	one year

 

Synonyms:

DTT-MBG;DTT (RACEMIC);DL-THREO-2,3-DIHYDROXY-1,4-DITHIOLBUTANE;DL-THREO-1,4-DIMERCAPTO-2,3-BUTANEDIOL;DL-DITHIOTHREITOL;DL-DTT;DITHIOTHREITOL, 1,4-;DITT;Clelands REDUCTACRYL; threo-1,4-Dimercapto-2,3-butanediol; 1,4-dithio-dl-threitol sol.; 1,4-Dithio-DL-threitol; dithiothreitol (dtt) sequencing grade; dithiothreitol molecular biology*reagent; DithiothreitollelandsReagenz; Dithithreitol; DL-Dithothreitol; (2S,3S)-1,4-disulfanylbutane-2,3-diol; DTT; (R,R)-Dithiothreitol; ; D-threo-1,4-Dimercapto-2,3-butanediol; 2,3-Butanediol, 1,4-dimercapto-, D-threo-; Cleland reagent; D-1,4-Dithiothreitol; D-Dtt; Sputolysin; Threitol, 1,4-dithio-; (R*,R*)-1,4-Dimercapto-2,3-butanediol; 1,4-Dithio-l-threitol; Cleland's reagent; Cleland regent; Clelands reagent; L-DTT; DL-1,4-Dithiothreitol; TPTZ; 2,4,6-Tri(2-pyridyl)-1,3,5-triazine

 

Related categories:

Biochemistry;O-Substituted Sugars;Sugar Alcohols;Sugars;Reagents for Electrophoresis

 

Related Products:

 

The determination of PH

Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, determination of the pH used have been calibrated pH meter.

 

The determination of solubility

Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, observe the solution.

 

Determination of melting point

   Instrument:

   Micro melting point apparatus WRS-2A, Melting point tubes

  Determination of step:

Open switch, stable 20min.

First set the starting temperature than the melting point of the low 10 ℃, heating rate 2 ℃ / min.

The sample was grind as fine as possible and load in a clean, dry melting point pipe ,until the sample was compressed into about 1cm high .When the actual temperature reaches the set temperature and stabilized . Inserted into the capillary with the sample, the simultaneous determination of three.

 

Detection of heavy metals

     The meaning of heavy metals of this act is under the specified experimental condition, which can coloration effects with sodium sulfide or thioacetamide  .The glasses of preparation or storage is not allowed to contain lead.

5.8.1  Reagents

  a) Lead dinitrate:1μg/mL

   b) Sodium hydroxide :10%(m/v)

   c) Sodium sulfide :10%(m/v)

5.8.2  Preparation of lead standard solution

     Weigh lead dinitrate  0.1599g ,relocated to 1000mL volumetric flask, plus nitric acid 5mL and water 50mL, diluted with water to the mark , shake, as the stock solution.

5.8.3 Determination of step

   Before use, the exact amount of stock solution 1mL, relocated to a 100ml volumetric flask, dilute with water to the mark, shake, limit the use of the day .

   Weigh sample 1g, after adding sodium hydroxide solution 5mL and 20mL of water to dissolve, set Nessler colorimetric tube, add 5 drops of sodium sulfide , shake , compared the color with 5mL lead standard solution after the same treatment,  not deeper

 

The determination of UV absorbance

  A certain amount of the sample was weighed to the nearest 0.0001g, into 10ml volumetric flask